Recombinant human CCL1/I-309 protein
CCL1 (chemokine C-C motif ligand 1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. unlike the other members of the C-C family, CCL1 does not induce chemotaxis in natural killer (NK) cells. Recombinant human CCL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 85% by SDS-PAGE
Molecular Weight
10.8 kDa (94aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Miller M.D., et al. (1992) Proc. Natl. Acad. Sci. u.S.A. 89:2950-2954 ; ; Roos RS, et al. (1997) J. Biol. Chem. 272 (28) : 17251-4.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM DTT, 10% glycerol, 50 mM NaCl
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_002972
Uniprot Accession Number
P22362
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK
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