Recombinant human EF-1 beta/EEF1B2 protein
Elongation factor 1-beta, also known as EEF1B2, is a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. EEF1B2 and EEF1D stimulate the exchange of GDP bound to EEF1A to GTP. Recombinant human EEF1B2 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Elongation factor 1-beta, EF-1-beta, EEF1B, EEF1B1, EF1B, Eukaryotic translation elongation factor 1 beta 2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
25.8 kDa (233aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Byun HO., et al. (2009) Cancer Res, 69 (11) :4638-47.; ; Sang Lee J., et al. (2002) Biochem Biophys Res Commun. 291 (1) :158-64.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 100 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_066944
Uniprot Accession Number
P24534
Species
Human
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