Recombinant human CDC25A protein
M-phase inducer phosphatase 1, also known as CDC25A, is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Recombinant human CDC25A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 80% by SDS-PAGE
Molecular Weight
63.2 kDa (560aa)
Additionnal Information
References & Citations
Pereg Y., et al. (2010) Nat. Cell Biol. 12:400-406; ; Soerensen C.S., et al. (2003) Cancer Cell 3:247-258
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 20% glycerol, 0.2M NaCl, 1mM EDTA
Scientific Category
Cell Cycle
NCBI Accession Number
NP_001780
Uniprot Accession Number
P30304
Species
Human
AA Sequence
Frequently Asked Questions
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