Recombinant human CDC25A protein

M-phase inducer phosphatase 1, also known as CDC25A, is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Recombinant human CDC25A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, Cdc 25a, Cell division cycle 25 homolog A (S. pombe), Cell division cycle 25A, Cell division cycle 25A isoform a, Cell division cycle 25A isoform b, D9Ertd393e, EC 3.1.3.48, M phase inducer phosphatase 1, MGC115549

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

63.2 kDa (560aa)

Additionnal Information

CDC25A, M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, Cdc 25a, Cell division cycle 25 homolog A (S. pombe), Cell division cycle 25A, Cell division cycle 25A isoform a, Cell division cycle 25A isoform b, D9Ertd393e, EC 3.1.3.48, M phase inducer phosphatase 1, MGC115549, ATGP0945-10 µg, ATGP0945-20 µg, ATGP0945-50 µg, ATGP0945-100 µg, ATGP0945-250 µg, ATGP0945-500 µg, ATGP0945-1 mg, ATGP0945-10, ATGP0945-20, ATGP0945-50, ATGP0945-100, ATGP0945-250, ATGP0945-500, ATGP0945-1

References & Citations

Pereg Y., et al. (2010) Nat. Cell Biol. 12:400-406; ; Soerensen C.S., et al. (2003) Cancer Cell 3:247-258

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 20% glycerol, 0.2M NaCl, 1mM EDTA

Scientific Category

Cell Cycle

NCBI Accession Number

NP_001780

Uniprot Accession Number

P30304

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMELG PEPPHRRRLL FACSPPPASQ PVVKALFGAS AAGGLSPVTN LTVTMDQLQG LGSDYEQPLE VKNNSNLQRM GSSESTDSGF CLDSPGPLDS KENLENPMRR IHSLPQKLLG CSPALKRSHS DSLDHDIFQL IDPDENKENE AFEFKKPVRP VSRGCLHSHG LQEGKDLFTQ RQNSAPARML SSNERDSSEP GNFIPLFTPQ SPVTATLSDE DDGFVDLLDG ENLKNEEETP SCMASLWTAP LVMRTTNLDN RCKLFDSPSL CSSSTRSVLK RPERSQEESP PGSTKRRKSM SGASPKESTN PEKAHETLHQ SLSLASSPKG TIENILDNDP RDLIGDFSKG YLFHTVAGKH QDLKYISPEI MASVLNGKFA NLIKEFVIID CRYPYEYEGG HIKGAVNLHM EEEVEDFLLK KPIVPTDGKR VIVVFHCEFS SERGPRMCRY VRERDRLGNE YPKLHYPELY VLKGGYKEFF MKCQSYCEPP SYRPMHHEDF KEDLKKFRTK SRTWAGEKSK REMYSRLKKL