Recombinant human BCKDHA protein

BCKDHA (branched-chain alpha-keto acid dehydrogenase E1 component alpha chain), also known as 2-oxoisovalerate dehydrogenase subunit alpha, belongs to the BCKDHA family. The branched-chain alpha-keto acid dehydrogenase complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3) . Recombinant human BCKDHA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

2-oxoisovalerate dehydrogenase subunit alpha, BCKDE1A, BCKDH E1-alpha, MSu, MSuD1, OVD1A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

47.8 kDa (421aa) confirmed by MALDI-TOF

Additionnal Information

BCKDHA, 2-oxoisovalerate dehydrogenase subunit alpha, BCKDE1A, BCKDH E1-alpha, MSu, MSuD1, OVD1A, ATGP0944-10 µg, ATGP0944-20 µg, ATGP0944-50 µg, ATGP0944-100 µg, ATGP0944-250 µg, ATGP0944-500 µg, ATGP0944-1 mg, ATGP0944-10, ATGP0944-20, ATGP0944-50, ATGP0944-100, ATGP0944-250, ATGP0944-500, ATGP0944-1

References & Citations

Nobukuni Y., et al. (1993) Biochim. Biophys. Acta 1225:64-70; ; Funchal, C., et al. (2005) Metab. Brain Dis. 20: 205-217

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 0.2M NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_000700.1

Uniprot Accession Number

P12694

Species

Human

AA Sequence

MSSLDDKPQF PGASAEFIDK LEFIQPNVIS GIPIYRVMDR QGQIINPSED PHLPKEKVLK LYKSMTLLNT MDRILYESQR QGRISFYMTN YGEEGTHVGS AAALDNTDLV FGQYREAGVL MYRDYPLELF MAQCYGNISD LGKGRQMPVH YGCKERHFVT ISSPLATQIP QAVGAAYAAK RANANRVVIC YFGEGAASEG DAHAGFNFAA TLECPIIFFC RNNGYAISTP TSEQYRGDGI AARGPGYGIM SIRVDGNDVF AVYNATKEAR RRAVAENQPF LIEAMTYRIG HHSTSDDSSA YRSVDEVNYW DKQDHPISRL RHYLLSQGWW DEEQEKAWRK QSRRKVMEAF EQAERKPKPN PNLLFSDVYQ EMPAQLRKQQ ESLARHLQTY GEHYPLDHFD K