Recombinant human UFM1 protein
UFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme UBA5 and E2-like conjugating enzyme UFC1 in a manner analogous to ubiquitylation. It localizes primarily to the nucleus, but is also present in diffuse amounts in the cytoplasm. This protein is expressed in a variety of tissues, including kidney, brain, heart, liver and lung. Recombinant human UFM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ubiquitin-fold modifier 1, bA131P10.1, BM-002, C13orf20
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by BCA assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
11.1 kDa (103aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Sasakawa H., et al. (2006) Biochem Biophys Res Commun. 343 (1) :21-6. ; ; Komatsu M., et al. (2004) EMBO J. 23 (9) :1977-86.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Ubiquitination
NCBI Accession Number
NP_057701.1
Uniprot Accession Number
P61960
Species
Human
AA Sequence
MSKVSFKITL TSDPRLPYKV LSVPESTPFT AVLKFAAEEF KVPAATSAII TNDGIGINPA QTAGNVFLKH GSELRIIPRD RVG
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