Recombinant human TDO2 protein

TDO2, also known as TDO, is a ferrous heme enzyme that catalyzes the first and rate-limiting step in the kynurenine pathway, the major pathway of tryptophan metabolism. It incorporates oxygen into the indole moiety of tryptophan. It has a broad specificity towards tryptamine and derivatives including D- and L-tryptophan, 5-hydroxytryptophan and serotonin. Recombinant human TDO2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tryptophan 23-dioxygenase, Tryptophan 2,3-dioxygenase, TDO, TPH2, TRPO

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

50 kDa (426aa) confirmed by MALDI-TOF

Additionnal Information

TDO2, Tryptophan 23-dioxygenase, Tryptophan 2,3-dioxygenase, TDO, TPH2, TRPO, ATGP0939-10 µg, ATGP0939-20 µg, ATGP0939-50 µg, ATGP0939-100 µg, ATGP0939-250 µg, ATGP0939-500 µg, ATGP0939-1 mg, ATGP0939-10, ATGP0939-20, ATGP0939-50, ATGP0939-100, ATGP0939-250, ATGP0939-500, ATGP0939-1

References & Citations

Murray MF., et al. (2007) Curr Drug Metab. 8 (3) :197-200.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_005642

Uniprot Accession Number

P48775

Species

Human

AA Sequence

MSGCPFLGNN FGYTFKKLPV EGSEEDKSQT GVNRASKGGL IYGNYLHLEK VLNAQELQSE TKGNKIHDEH LFIITHQAYE LWFKQILWEL DSVREIFQNG HVRDERNMLK VVSRMHRVSV ILKLLVQQFS ILETMTALDF NDFREYLSPA SGFQSLQFRL LENKIGVLQN MRVPYNRRHY RDNFKGEENE LLLKSEQEKT LLELVEAWLE RTPGLEPHGF NFWGKLEKNI TRGLEEEFIR IQAKEESEEK EEQVAEFQKQ KEVLLSLFDE KRHEHLLSKG ERRLSYRALQ GALMIYFYRE EPRFQVPFQL LTSLMDIDSL MTKWRYNHVC MVHRMLGSKA GTGGSSGYHY LRSTVSDRYK VFVDLFNLST YLIPRHWIPK MNPTIHKFLY TAEYCDSSYF SSDESD