Recombinant human Transgelin 2/TAGLN2 protein

TAGLN2, also known transgelin 2, is one of the earliest markers of differentiated smooth muscle. This protein contains a calponin like repeat and a calponin-homology (CH) domain. It is downregulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. It also binds actin, causing actin fibers to gel within minutes of binding. Binding of transgelin to actin occurs at a ratio of 1:6 actin monomers. Recombinant human TAGLN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Transgelin-2, HA1756, KIAA0120, Transgelin 2, SM22-alpha homolog, Epididymis tissue protein Li 7e

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.4 kDa (208aa) confirmed by MALDI-TOF

Additionnal Information

TAGLN2, Transgelin-2, HA1756, KIAA0120, Transgelin 2, ATGP0938-10 µg, ATGP0938-20 µg, ATGP0938-50 µg, ATGP0938-100 µg, ATGP0938-250 µg, ATGP0938-500 µg, ATGP0938-1 mg, ATGP0938-10, ATGP0938-20, ATGP0938-50, ATGP0938-100, ATGP0938-250, ATGP0938-500, ATGP0938-1

References & Citations

Shapland C, et al. (1993) . J. Cell Biol., 121:1065-1073.; ; Kobayashi R, et al. (1994) . Biochem. Biophys. Res. Commun. 198:1275-1280.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Development

NCBI Accession Number

NP_003555

Uniprot Accession Number

P37802

Species

Human

AA Sequence

EVQQKIEKQ YDADLEQILI QWITTQCRKD VGRPQPGREN FQNWLKDGTV LCELINALYP EGQAPVKKIQ ASTMAFKQME QISQFLQAAE RYGINTTDIF QTVDLWEGKN MACVQRTLMN LGGLAVARDD GLFSGDPNWF PKKSKENPRN FSDNQLQEGK NVIGLQMGTN RGASQAGMTG YGMPRQIL