Recombinant human NUDT21/CPSF5 protein

NuDT21, also known as CPSF5 or CFIm25, is a member of the Nudix hydrolase family of pyrophosphatases. This protein localizes to the paraspeckles and forms a heterodimer with CPSF6 or CPSF7 to comprise the CFIm (mammalian cleavage factor I) complex. NuDT21 is the smaller subunit of the complex and is present in all heterodimer combinations. NuDT21 plays an important role in pre-mRNA 3' cleavage and polyadenylation processing. Recombinant human NuDT21 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nudix hydrolase 21, Cleavage and polyadenylation specificity factor subunit 5, Cleavage and polyadenylation specificity factor 25 kDa subunit, CPSF 25 kDa subunit, Cleavage factor Im complex 25 kDa subunit nudix-type motif 21, CFIM25, Cleavage factor Im complex 25 kDa subunit, Nucleoside diphosphate-linked moiety X motif 21, Nudix motif 21, Pre-mRNA cleavage factor Im 68 kDa subunit, CPSF25

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

28.3 kDa (247aa) confirmed by MALDI-TOF

Additionnal Information

NUDT21, Nudix (nucleoside diphosphate linked moiety X) -type motif 21, CFIM25, CPSF5, DKFZp686H1588, ATGP0935-10 µg, ATGP0935-20 µg, ATGP0935-50 µg, ATGP0935-100 µg, ATGP0935-250 µg, ATGP0935-500 µg, ATGP0935-1 mg, ATGP0935-10, ATGP0935-20, ATGP0935-50, ATGP0935-100, ATGP0935-250, ATGP0935-500, ATGP0935-1

References & Citations

Kim S., et al. (2010) Genes Cells. 15 (9) :1003-13.; ; Shimazu T., et al. (2007) J Biol Chem. 282 (7) :4470-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 2mM DTT, 20% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_008937

Uniprot Accession Number

O43809

Species

Human

AA Sequence

MSVVPPNRSQ TGWPRGVTQF GNKYIQQTKP LTLERTINLY PLTNYTFGTK EPLYEKDSSV AARFQRMREE FDKIGMRRTV EGVLIVHEHR LPHVLLLQLG TTFFKLPGGE LNPGEDEVEG LKRLMTEILG RQDGVLQDWV IDDCIGNWWR PNFEPPQYPY IPAHITKPKE HKKLFLVQLQ EKALFAVPKN YKLVAAPLFE LYDNAPGYGP IISSLPQLLS RFNFIYN