Recombinant e.coli fimC protein

The 23 kDa two-domain periplasmic chaperone FIMC from Escherichia coli is required for the assembly of type-1 pili, which are filamentous, highly oligomeric protein complexes anchored to the outer bacterial membrane that mediate adhesion of pathogenic E. coli strains to host cell surfaces and persist in macrophages. Recombinant E. coli FIMC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Periplasmic chaperone required for type 1 fimbriae, Periplasmic chaperone, required for type 1 fimbriae, ECK4307, JW4279

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

25 kDa (226aa) confirmed by MALDI-TOF

Additionnal Information

FimC, Periplasmic chaperone required for type 1 fimbriae, Periplasmic chaperone, required for type 1 fimbriae, ECK4307, JW4279, ATGP0931-10 µg, ATGP0931-20 µg, ATGP0931-50 µg, ATGP0931-100 µg, ATGP0931-250 µg, ATGP0931-500 µg, ATGP0931-1 mg, ATGP0931-10, ATGP0931-20, ATGP0931-50, ATGP0931-100, ATGP0931-250, ATGP0931-500, ATGP0931-1

References & Citations

Hermanns u., et al. (2000) Biochemistry. 39 (38) :11564-70.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_418736

Uniprot Accession Number

P31697

Species

E.coli

AA Sequence

GVALGATRV IYPAGQKQEQ LAVTNNDENS TYLIQSWVEN ADGVKDGRFI VTPPLFAMKG KKENTLRILD ATNNQLPQDR ESLFWMNVKA IPSMDKSKLT ENTLQLAIIS RIKLYYRPAK LALPPDQAAE KLRFRRSANS LTLINPTPYY LTVTELNAGT RVLENALVPP MGESTVKLPS DAGSNITYRT INDYGALTPK MTGVME