Recombinant human MASA/ENOPH1 protein

Enolase-phosphatase E1, also known as ENOPH1, is a member of the MasA family of the HAD (halo-acid dehalogenase) -like hydrolase superfamily. ENOPH1 is a bifunctional enzyme, exhibiting both phosphatase and atypical enolase activities. ENOPH1 plays an important role in the ubiquitous methionine salvage pathway, a biochemical pathway found in all organisms that regulate methionine levels in the cell. Recombinant human ENOPH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Enolase-phosphatase E1, MASA, MST145, Acireductone synthase, 2,3-diketo-5-methylthio-1-phosphopentane phosphatase, E1, mtnC

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

31 kDa (281aa) confirmed by MALDI-TOF

Additionnal Information

ENOPH1, Enolase-phosphatase E1, MASA, MST145, ATGP0927-10 µg, ATGP0927-20 µg, ATGP0927-50 µg, ATGP0927-100 µg, ATGP0927-250 µg, ATGP0927-500 µg, ATGP0927-1 mg, ATGP0927-10, ATGP0927-20, ATGP0927-50, ATGP0927-100, ATGP0927-250, ATGP0927-500, ATGP0927-1

References & Citations

Zhang Y., et al. (2004) Bioorq Med Chem. 12:3847-3855.; ; Wang H., et al. (2005) Acta Crystallogr. 61:521-523.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_067027

Uniprot Accession Number

Q9UHY7

Species

Human

AA Sequence

MVVLSVPAEV TVILLDIEGT TTPIAFVKDI LFPYIEENVK EYLQTHWEEE ECQQDVSLLR KQAEEDAHLD GAVPIPAASG NGVDDLQQMI QAVVDNVCWQ MSLDRKTTAL KQLQGHMWRA AFTAGRMKAE FFADVVPAVR KWREAGMKVY IYSSGSVEAQ KLLFGHSTEG DILELVDGHF DTKIGHKVES ESYRKIADSI GCSTNNILFL TDVTREASAA EEADVHVAVV VRPGNAGLTD DEKTYYSLIT SFSELYLPSS T