Recombinant human LYPLA2 protein
Acyl-protein thioesterase 2, also known LYPLA2, is lysophospholipase that acts on biological membranes to regulate the multifunctional lysophospholipids. This protein may hydrolyze fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Recombinant human LYPLA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Acyl-protein thioesterase 2, APT-2, APT2, Lysophospholipase II, LPL-II, LysoPLA II
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
26.9 kDa (251aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Fan J., et al. (2008) Int J Infect Dis. 12 (2) :143-51.; ; Wanq A., et al. (2000) Biochim Biophys Acta. 1484:207-14.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol,100 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_009191.1
Uniprot Accession Number
O95372
Species
Human
AA Sequence
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