Recombinant human RPS14 protein

Ribosomal protein S14, also known as RPS14, is a ribosomal protein. Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit and a large subunit that consist of over 80 distinct ribosomal proteins. RPS14 is a component of the 40S subunit that localized to the cytoplasm. Recombinant human RPS14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

40S ribosomal protein S14, EMTB

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

18.7 kDa (174aa) confirmed by MALDI-TOF

Additionnal Information

RPS14, 40S ribosomal protein S14, EMTB, ATGP0925-10 µg, ATGP0925-20 µg, ATGP0925-50 µg, ATGP0925-100 µg, ATGP0925-250 µg, ATGP0925-500 µg, ATGP0925-1 mg, ATGP0925-10, ATGP0925-20, ATGP0925-50, ATGP0925-100, ATGP0925-250, ATGP0925-500, ATGP0925-1

References & Citations

Martin Nieto J., et al. (1997) J Cell Sci. 110:955-963.; ; Fiqueroa P., et al. (2000) Biochem Biophys Res Commun. 271:380-385.

Other References

Wang L, et al. Ribosomal protein S14 silencing inhibits growth of acute myeloid leukemia transformed from myelodysplastic syndromes via activating p53. (Hematology. 2014) {https://pubmed.ncbi.nlm.nih.gov/24074450/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 100 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_001020242.1

Uniprot Accession Number

P62263

Species

Human

AA Sequence

MAPRKGK EKKEEQVISL GPQVAEGENV FGVCHIFASF NDTFVHVTDL SGKETICRVT GGMKVKADRD ESSPYAAMLA AQDVAQRCKE LGITALHIKL RATGGNRTKT PGPGAQSALR ALARSGMKIG RIEDVTPIPS DSTRRKGGRR GRRL