Recombinant human MYL9 protein
MYL9, also known as MLC2, is one of the numerous regulatory myosin light chains. Regulatory myosin light chains regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK) . Phosphorylation of regulatory myosin light chains is catalyzed by MLCK in the presence of calcium and calmodulin and it increases the actin-activated myosin ATPase activity, thereby regulates the contractile activity. Recombinant human MYL9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
21.9 kDa (192aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Gilles L., et al. (2009) Blood. 114 (19) :4221-32.; ; Medjkane S., et al. (2009) Nat Cell Biol. 11 (3) :257-68.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_006088
Uniprot Accession Number
P24844
Species
Human
AA Sequence
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