Recombinant human MYL9 protein

MYL9, also known as MLC2, is one of the numerous regulatory myosin light chains. Regulatory myosin light chains regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK) . Phosphorylation of regulatory myosin light chains is catalyzed by MLCK in the presence of calcium and calmodulin and it increases the actin-activated myosin ATPase activity, thereby regulates the contractile activity. Recombinant human MYL9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Myosin light chain 9, Myosin regulatory light polypeptide 9, 20 kDa myosin light chain, LC20, MLC-2C, Myosin RLC, Myosin regulatory light chain 2 smooth muscle isoform, Myosin regulatory light chain 9, Myosin regulatory light chain MRLC1, MLC2, MYRL2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

21.9 kDa (192aa) confirmed by MALDI-TOF

Additionnal Information

MYRL2, MYRL 2, Myosin RLC, Myosin regulatory light polypeptide9, Myosin regulatory light polypeptide 9, Myosin regulatory light chain MRLC1, Myosin regulatory light chain 9, Myosin regulatory light chain 2 smooth muscle isoform, Myosin light chain 9, MYL9, MRLC 1, MLC-2C, MLC2, MLC 2, MGC 3505, M RLC 1, LC20, LC 20, 20kDa myosin light chain, 20 kDa myosin light chain, ATGP0923-10 µg, ATGP0923-20 µg, ATGP0923-50 µg, ATGP0923-100 µg, ATGP0923-250 µg, ATGP0923-500 µg, ATGP0923-1 mg, ATGP0923-010, ATGP0923-020, ATGP0923-050, ATGP0923-100, ATGP0923-250, ATGP0923-500, ATGP0923-01M

References & Citations

Gilles L., et al. (2009) Blood. 114 (19) :4221-32.; ; Medjkane S., et al. (2009) Nat Cell Biol. 11 (3) :257-68.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_006088

Uniprot Accession Number

P24844

Species

Human

AA Sequence

MSSKRAKAKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID KEDLHDMLAS LGKNPTDEYL EGMMSEAPGP INFTMFLTMF GEKLNGTDPE DVIRNAFACF DEEASGFIHE DHLRELLTTM GDRFTDEEVD EMYREAPIDK KGNFNYVEFT RILKHGAKDK DD