Recombinant human NUDT3/DIPP1 protein
NuDT3, also known as DIPP, DIPP1 (diphosphoinositol polyphosphate phosphohydrolase 1), is a 172 amino acid cytoplasmic protein belonging to the nudix hydrolase family and DIPP subfamily. NuDT3 acts as a negative regulator of the ERK 1/2 pathway and hydrolyzes 5-phosphoribose 1-diphosphate. NuDT3 exists as a monomer and binds magnesium as a cofactor. Also NuDT3 is widely expressed but found at highest levels in liver, pancreas, brain and heart. Recombinant human NuDT3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Nudix hydrolase 3, Diadenosine 5',5'''-P1, P6-hexaphosphate hydrolase 1, Diphosphoinositol polyphosphate phosphohydrolase 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
21.6 kDa (192aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Chu C., et al. (2004) Cell Signal. 16 (9) :1045-59.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_006694
Uniprot Accession Number
O95989
Species
Human
AA Sequence
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