Recombinant human SF3B14A/SF3B6 protein

SF3B14, also known as SAP14, is a 125 amino acid nuclear protein that is a component of the splicing factor 3b complex. Splicing factor 3b associates with both the u2 and u11/u12 small nuclear ribonucleoprotein complexes (u2 snRNP) of spliceosomes. Required for the splicing of pre-mRNA, SF3B14 enters the spliceosome and associates with the pre-mRNA branch site facilitating the interaction of snRNP with the branch sites of u2 and u12 of the 17S u2 and the 18S u11/u12 snRNP complex. Recombinant human SF3B14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

"Splicing factor 3b subunit 6, SF3b 14 kDa subunit, Spliceosome-associated protein 14-kDa, Splicing factor 3b subunit 6 14kDa, pre mRNA branch site protein p14, P14, SF3B14a, Ht006, CGI-110, SAP14a, SAP14, SF3B14 "

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

16.7 kDa (145aa) confirmed by MALDI-TOF

Additionnal Information

SF3B14, Splicing factor 3B 14 kDa subunit, Splicing factor 3B, 14 kDa subunit, CGI-110, HSPC175, Ht006, P14, SAP14, SF3B14a, ATGP0920-10 µg, ATGP0920-20 µg, ATGP0920-50 µg, ATGP0920-100 µg, ATGP0920-250 µg, ATGP0920-500 µg, ATGP0920-1 mg, ATGP0920-10, ATGP0920-20, ATGP0920-50, ATGP0920-100, ATGP0920-250, ATGP0920-500, ATGP0920-1

References & Citations

Golas MM., et al. (2003) Science. 300 (5621) :980-4.; ; Will CL., et al. (2002) EMBO J. 21 (18) :4978-88.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol

Scientific Category

Other

NCBI Accession Number

NP_057131

Uniprot Accession Number

Q9Y3B4

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVVL YYNANRAFQK MDTKKKEEQL KLLKEKYGIN TDPPK