Recombinant human SNRPE protein
SNRPE, also known as SME, aids in the cytoplasmic construction of the usnRNPs by binding to a conserved Sm site on usnRNA and forming a stable snRNP core complex. As a core protein to usnRNP, the SNRPE associates with the entire u family of snRNAs including u1-u6. SNRPE also interacts with DDX20 and Small nuclear ribonucleoprotein polypeptide F. Recombinant human SNRPE protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Small nuclear ribonucleoprotein E, B-raf, Sm-E, SME
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
12.9 kDa (112aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Campion Y., et al. (2010) EMBO J. 29 (11) :1817-29.; ; Dong X., et al. (2009) Nature. 458 (7242) :1136-41.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol
Scientific Category
Other
NCBI Accession Number
NP_003085
Uniprot Accession Number
P62304
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MAYRGQGQKV QKVMVQPINL IFRYLQNRSR IQVWLYEQVN MRIEGCIIGF DEYMNLVLDD AEEIHSKTKS RKQLGRIMLK GDNITLLQSV SN
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