Recombinant human CCL3L1/LD78-beta protein
CCL3L1 is a small cytokine belonging to the CC chemokine. This protein is involved in immunoregulatory and inflammatory processes. It binds to several chemokine receptors including chemokine binding protein 2 (CCBP2 or D6) and chemokine (C-C motif) receptor 5 (CCR5) . Recombinant human CCL3L1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
10 kDa (90aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hirashima M, et al. (1992) DNA Seq. 3:203-12.; ; Miyakawa, et al. (2002) J. Biol. Chem. 277:4649-55.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_066286
Uniprot Accession Number
P16619
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MSLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPSV IFLTKRGRQV CADPSEEWVQ KYVSDLELSA
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