Recombinant human BLNK protein

BLNK, also known as B-cell linker, is essential for normal B-cell development. This protein has been shown to associate with the effector proteins GRB2, Vav, NCK and PLC gamma following activation of the B cell receptor. It is phosphorylated by the Syk tyrosine kinase, which in turn permits activation of downstream effector proteins including GRB2 and PLC gamma. Recombinant human BLNK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

B-cell linker, BASH, BLNK-S, LY57, MGC111051, SLP-65, SLP65

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

52.6 kDa (476aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

BLNK, B-cell linker, BASH, BLNK-S, LY57, MGC111051, SLP-65, SLP65, ATGP0915-10 µg, ATGP0915-20 µg, ATGP0915-50 µg, ATGP0915-100 µg, ATGP0915-250 µg, ATGP0915-500 µg, ATGP0915-1 mg, ATGP0915-10, ATGP0915-20, ATGP0915-50, ATGP0915-100, ATGP0915-250, ATGP0915-500, ATGP0915-1

References & Citations

Kurosaki T, et al. (1997) Curr. Opin. Immunol., 9:309-318.; ; Fu C, et al. (1998) . Immunity. 9:93-103.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF

Scientific Category

Signal Transduction

NCBI Accession Number

AAH18906.1

Uniprot Accession Number

Q8WV28

Species

Human

AA Sequence

MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMNKIKKLK VKAPPSVPRR DYASESPADE EQQWSDDFDS DYENPDEHSD SEMYVMPAEE NADDSYEPPP VEQETRPVHP ALPFARGEYI DNRSSQRHSP PFSKTLPSKP SWPSEKARLT STLPALTALQ KPQVPPKPKG LLEDEADYVV PVEDNDENYI HPTESSSPPP EKAPMVNRST KPNSSTPASP PGTASGRNSG AWETKSPPPA APSPLPRAGK KPTTPLKTTP VASQQNASSV CEEKPIPAER HRGSSHRQEA VQSPVFPPAQ KQIHQKPIPL PRFTEGGNPT VDGPLPSFSS NSTISEQEAG VLCKPWYAGA CDRKSAEEAL HRSNKDGSFL IRKSSGHDSK QPYTLVVFFN KRVYNIPVRF IEATKQYALG RKKNGEEYFG SVAEIIRNHQ HSPLVLIDSQ NNTKDSTRLK YAVKVS