Recombinant mouse Dihydrofolate reductase/DHFR protein

Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. DHFR catalyzes the NADPH-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX) -resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive. Recombinant mouse DHFR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.

Product Name Alternative

Dihydrofolate reductase, EC 1.5.1.3

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 2000pmol/min/ug, and is defined as the amount of enzyme that converts 1.0pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.

Molecular Weight

23.8 kDa (207aa) confirmed by MALDI-TOF

Additionnal Information

DHFR, Dihydrofolate reductase, EC 1.5.1.3, ATGP0912-10 µg, ATGP0912-20 µg, ATGP0912-50 µg, ATGP0912-100 µg, ATGP0912-250 µg, ATGP0912-500 µg, ATGP0912-1 mg, ATGP0912-10, ATGP0912-20, ATGP0912-50, ATGP0912-100, ATGP0912-250, ATGP0912-500, ATGP0912-1

References & Citations

Chen MJ., et al. (1984) J Biol Chem. 259 (6) :3933-43. ; ; Cody V., et al. (2009) Biochemistry. 48 (8) :1702-11.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.

Scientific Category

Redox Proteins

NCBI Accession Number

NP_034179.1

Uniprot Accession Number

P00375

Species

Mouse

AA Sequence

MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD