Recombinant human Lipocalin-2/NGAL protein

Neutrophil gelatinase-associated lipocalin (NGAL), also known as LCN2, belongs to the calycin superfamily and Lipocalin family. LCN2 is an iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. The binding of LCN2 to bacterial siderophores is important in the innate immune response to bacterial infection. Also LCN2 functions as a growth factor. LCN2 is strongly upregulated during inflammation and is upregulated by interleukin 1 (but not TNF alpha) in humans. Recombinant human LCN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Neutrophil gelatinase-associated lipocalin, Lipocalin-2, MSFI, NGAL, Oncogene 24p3, p25, HNL

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

22.8 kDa (199aa) confirmed by MALDI-TOF

Additionnal Information

Lipocalin2, NGAL, Neutrophil gelatinase-associated lipocalin, Lipocalin-2, MSFI, NGAL, Oncogene 24p3, p25, HNL, ATGP0908-10 µg, ATGP0908-20 µg, ATGP0908-50 µg, ATGP0908-100 µg, ATGP0908-250 µg, ATGP0908-500 µg, ATGP0908-1 mg, ATGP0908-10, ATGP0908-20, ATGP0908-50, ATGP0908-100, ATGP0908-250, ATGP0908-500, ATGP0908-1

References & Citations

Flo TH., et al. (2004) Nature 432 (7019) : 917-21; ; Yang J., et al. (2002) Mol. Cell 10:1045-1056

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Scientific Category

Immunology

NCBI Accession Number

NP_005555

Uniprot Accession Number

P80188

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MQDSTSDLIP APPLSKVPLQ QNFQDNQFQG KWYVVGLAGN AILREDKDPQ KMYATIYELK EDKSYNVTSV LFRKKKCDYW IRTFVPGCQP GEFTLGNIKS YPGLTSYLVR VVSTNYNQHA MVFFKKVSQN REYFKITLYG RTKELTSELK ENFIRFSKSL GLPENHIVFP VPIDQCIDG