Recombinant human MLF1 protein

MLF1 (Myeloid leukemia factor 1) belongs to MLF family, and is a widely expressed negative regulator of cell cycle progression functioning upstream of the tumor suppressor p53. This protein interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. MLF1 normally functions in multi-potent progenitor cells, and its dysregulation may be somewhat responsible for leukemogenesis. Recombinant human MLF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Myeloid leukemia factor 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

32.7 kDa (288aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

MLF1, Myeloid leukemia factor 1, ATGP0906-10 µg, ATGP0906-20 µg, ATGP0906-50 µg, ATGP0906-100 µg, ATGP0906-250 µg, ATGP0906-500 µg, ATGP0906-1 mg, ATGP0906-10, ATGP0906-20, ATGP0906-50, ATGP0906-100, ATGP0906-250, ATGP0906-500, ATGP0906-1

References & Citations

Yoneda-Kato N., et al. (2005) EMBO J. 24:1739-1749; ; Winteringham L.N., et al (2004) Oncogene 23: 5105-5109

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 5mM DTT, 200 mM NaCl

Scientific Category

Cell Cycle

NCBI Accession Number

NP_071888

Uniprot Accession Number

P58340

Species

Human

AA Sequence

MFRMLNSSFE DDPFFSESIL AHRENMRQMI RSFSEPFGRD LLSISDGRGR AHNRRGHNDG EDSLTHTDVS SFQTMDQMVS NMRNYMQKLE RNFGQLSVDP NGHSFCSSSV MTYSKIGDEP PKVFQASTQT RRAPGGIKET RKAMRDSDSG LEKMAIGHHI HDRAHVIKKS KNKKTGDEEV NQEFINMNES DAHAFDEEWQ SEVLKYKPGR HNLGNTRMRS VGHENPGSRE LKRREKPQQS PAIEHGRRSN VLGDKLHIKG SSVKSNKK