Recombinant human MLF1 protein
MLF1 (Myeloid leukemia factor 1) belongs to MLF family, and is a widely expressed negative regulator of cell cycle progression functioning upstream of the tumor suppressor p53. This protein interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. MLF1 normally functions in multi-potent progenitor cells, and its dysregulation may be somewhat responsible for leukemogenesis. Recombinant human MLF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Myeloid leukemia factor 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
32.7 kDa (288aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Yoneda-Kato N., et al. (2005) EMBO J. 24:1739-1749; ; Winteringham L.N., et al (2004) Oncogene 23: 5105-5109
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 5mM DTT, 200 mM NaCl
Scientific Category
Cell Cycle
NCBI Accession Number
NP_071888
Uniprot Accession Number
P58340
Species
Human
AA Sequence
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