Recombinant human PEF1 protein

Penta-EF-hand domain containing 1, also known as PEF1, belongs to the penta-EF-hand protein family, which shares similarities to the apoptosis linked gene ALG-2. PEF1 heterodimerizes with PDCD6/ALG2 and dissociates from PDCD6/ALG2 in the presence of Ca (2+) . Also, PEF1 proteins bind calcium and participate in a variety of calcium-dependent processes in vertebrates. Recombinant human PEF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Penta-EF-hand domain containing 1, ABP32, PEF1A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

34.5 kDa (320aa) confirmed by MALDI-TOF

Additionnal Information

PEF1, Penta-EF-hand domain containing 1, ABP32, PEF1A, ATGP0905-10 µg, ATGP0905-20 µg, ATGP0905-50 µg, ATGP0905-100 µg, ATGP0905-250 µg, ATGP0905-500 µg, ATGP0905-1 mg, ATGP0905-10, ATGP0905-20, ATGP0905-50, ATGP0905-100, ATGP0905-250, ATGP0905-500, ATGP0905-1

References & Citations

Vernarecci S., et al. (2007) Mol Microbiol. 65 (4) :1122-38.; ; Kitaura Y., et al. (2002) Arch Biochem Biophys. 399 (1) :12-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.2M NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_036524

Uniprot Accession Number

Q9UBV8

Species

Human

AA Sequence

MASY PYRQGCPGAA GQAPGAPPGS YYPGPPNSGG QYGSGLPPGG GYGGPAPGGP YGPPAGGGPY GHPNPGMFPS GTPGGPYGGA APGGPYGQPP PSSYGAQQPG LYGQGGAPPN VDPEAYSWFQ SVDSDHSGYI SMKELKQALV NCNWSSFNDE TCLMMINMFD KTKSGRIDVY GFSALWKFIQ QWKNLFQQYD RDRSGSISYT ELQQALSQMG YNLSPQFTQL LVSRYCPRSA NPAMQLDRFI QVCTQLQVLT EAFREKDTAV QGNIRLSFED FVTMTASRML