Recombinant human ATG5 protein
Autophagy protein 5, also known as ATG5, belongs to the autophagy-related (ATG) genes family. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. ATG5 protein is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Recombinant human ATG5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Autophagy protein 5, APG5, APG5-LIKE, APG5L, ASP, hAPG5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
34.6 kDa (295aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Mizushima N., et al. (1998) Nature. 395:395-8.; ; Tanida I., et al. (1999) Mol Biol Cell. 10:1367-79.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.2M NaCl
Scientific Category
Apoptosis
NCBI Accession Number
NP_004840
Uniprot Accession Number
Q9H1Y0
Species
Human
AA Sequence
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