Recombinant human eIF-1AY/EIF1AY protein
EIF1AY is similar to eukaryotic translation initiation factor 1A (EIF1A) . This protein seems to be required for maximal rate of protein biosynthesis. It enhances ribosome dissociation into subunits and is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. Recombinant human EIF1AY protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
18.8 kDa (167aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Mahbybyl Huq AH., et al. (2009) J Child Neurol. 24 (10) :1258-61; ; Page DC., et al (1997) Science. 278 (5338) :675-80.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 100 mM NaCl, 20% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_004672
Uniprot Accession Number
O14602
Species
Human
AA Sequence
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
