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Recombinant human eIF-1AY/EIF1AY protein

EIF1AY is similar to eukaryotic translation initiation factor 1A (EIF1A) . This protein seems to be required for maximal rate of protein biosynthesis. It enhances ribosome dissociation into subunits and is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. Recombinant human EIF1AY protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Eukaryotic translation initiation factor 1A Y-linked, Eukaryotic translation initiation factor 1A, Y-linked, eIF-1A Y isoform, eukaryotic translation initiation factor 4C (eIF-4C)

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

18.8 kDa (167aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

EIF1AY, Eukaryotic translation initiation factor 1A Y-linked, Eukaryotic translation initiation factor 1A, Y-linked, eIF-1A Y isoform, eukaryotic translation initiation factor 4C (eIF-4C), ATGP0902-10 µg, ATGP0902-20 µg, ATGP0902-50 µg, ATGP0902-100 µg, ATGP0902-250 µg, ATGP0902-500 µg, ATGP0902-1 mg, ATGP0902-10, ATGP0902-20, ATGP0902-50, ATGP0902-100, ATGP0902-250, ATGP0902-500, ATGP0902-1

References & Citations

Mahbybyl Huq AH., et al. (2009) J Child Neurol. 24 (10) :1258-61; ; Page DC., et al (1997) Science. 278 (5338) :675-80.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 100 mM NaCl, 20% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004672

Uniprot Accession Number

O14602

Species

Human

AA Sequence

MPKNKGK GGKNRRRGKN ENESEKRELV FKEDGQEYAQ VIKMLGNGRL EALCFDGVKR LCHIRGKLRK KVWINTSDII LVGLRDYQDN KADVILKYNA DEARSLKAYG ELPEHAKINE TDTFGPGDDD EIQFDDIGDD DEDIDDI

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