Recombinant human RAB1A protein

RAB1A, also known as ras-related protein Rab-1A, is a member of ras-related superfamily. Rab proteins play an important role for, either in endocytosis or in biosynthetic protein transport. Rab1a protein controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus. Recombinant human RAB1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Ras-related protein Rab-1A isoform 1, DKFZp564B163, RAB1, YPT1, RAB1A member RAS oncogene family, Rab GTPase YPT1, YPT1-related protein

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

24.8 kDa (225aa) confirmed by MALDI-TOF

Additionnal Information

RAB1A, ATGP0900-10 µg, ATGP0900-20 µg, ATGP0900-50 µg, ATGP0900-100 µg, ATGP0900-250 µg, ATGP0900-500 µg, ATGP0900-1 mg, ATGP0900-10, ATGP0900-20, ATGP0900-50, ATGP0900-100, ATGP0900-250, ATGP0900-500, ATGP0900-1

References & Citations

Zahraoui A, et al. (1989), J. Biol. Chem., 264: 12394-12401.; ; Chavrier P., et al. (1992) . Gene. 112: 261-264.

Other References

Wu R, et al. Baicalein Targets GTPase-Mediated Autophagy to Eliminate Liver Tumor-Initiating Stem Cell-Like Cells Resistant to mTORC1 Inhibition. (Hepatology. 2018) {https://pubmed.ncbi.nlm.nih.gov/29729190/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_004152

Uniprot Accession Number

P62820

Species

Human

AA Sequence

MSSMNPEYDY LFKLLLIGDS GVGKSCLLLR FADDTYTESY ISTIGVDFKI RTIELDGKTI KLQIWDTAGQ ERFRTITSSY YRGAHGIIVV YDVTDQESFN NVKQWLQEID RYASENVNKL LVGNKCDLTT KKVVDYTTAK EFADSLGIPF LETSAKNATN VEQSFMTMAA EIKKRMGPGA TAGGAEKSNV KIQSTPVKQS GGGCC