Recombinant human TFAM protein
TFAM, also known as MtTF1 or mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. TFAM is located primarily in the nuclei of elongated spermatids and may be involved in the regulation of gene expression of the haploid male genome. TFAM has been associated with mitochondrial disorder in humans characterized by ocular myopathy, exercise intolerance and muscle wasting. Recombinant human TFAM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Transcription factor A mitochondrial precursor, Transcription factor A, mitochondrial precursor, MtTF1, mtTFA, TCF6, TCF6L1, TCF6L2, TCF6L3
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
26.6 kDa (225aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Sinha RA., et al. (2010) Biochem Biophys Res Commun. 397 (3) :548-52.; ; Aquilano K., et al. (2010) J Biol Chem. 285 (28) :21590-9.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_003192.1
Uniprot Accession Number
Q00059
Species
Human
AA Sequence
Frequently Asked Questions
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