Recombinant human TFAM protein

TFAM, also known as MtTF1 or mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. TFAM is located primarily in the nuclei of elongated spermatids and may be involved in the regulation of gene expression of the haploid male genome. TFAM has been associated with mitochondrial disorder in humans characterized by ocular myopathy, exercise intolerance and muscle wasting. Recombinant human TFAM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Transcription factor A mitochondrial precursor, Transcription factor A, mitochondrial precursor, MtTF1, mtTFA, TCF6, TCF6L1, TCF6L2, TCF6L3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

26.6 kDa (225aa) confirmed by MALDI-TOF

Additionnal Information

TFAM, Transcription factor A mitochondrial precursor, Transcription factor A, mitochondrial precursor, MtTF1, mtTFA, TCF6, TCF6L1, TCF6L2, TCF6L3, ATGP0897-10 µg, ATGP0897-20 µg, ATGP0897-50 µg, ATGP0897-100 µg, ATGP0897-250 µg, ATGP0897-500 µg, ATGP0897-1 mg, ATGP0897-10, ATGP0897-20, ATGP0897-50, ATGP0897-100, ATGP0897-250, ATGP0897-500, ATGP0897-1

References & Citations

Sinha RA., et al. (2010) Biochem Biophys Res Commun. 397 (3) :548-52.; ; Aquilano K., et al. (2010) J Biol Chem. 285 (28) :21590-9.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_003192.1

Uniprot Accession Number

Q00059

Species

Human

AA Sequence

SSVLASCPK KPVSSYLRFS KEQLPIFKAQ NPDAKTTELI RRIAQRWREL PDSKKKIYQD AYRAEWQVYK EEISRFKEQL TPSQIMSLEK EIMDKHLKRK AMTKKKELTL LGKPKRPRSA YNVYVAERFQ EAKGDSPQEK LKTVKENWKN LSDSEKELYI QHAKEDETRY HNEMKSWEEQ MIEVGRKDLL RRTIKKQRKY GAEEC