Recombinant human DDIT4 protein
DDIT4, also known as Dig2 or REDD1, is thought to have function in the regulation of reactive oxygen species. In response to stress due to DNA damage and glucocorticoid treatment, DDIT4 is upregulated at the transcriptional level. DDIT4 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. Recombinant human DDIT4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
DNA damage-inducible transcript 4 protein, Dig2, FLJ20500, REDD1, RP11-442H21.1, RTP801
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
27.5 kDa (252aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Yoshida T., et al. (2010) Nat Med. 16 (7) :767-73.; ; Regazzetti C., et al. (2010) J Biol Chem. 285 (8) :5157-64.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol
Scientific Category
Cancer
NCBI Accession Number
NP_061931
Uniprot Accession Number
Q9NX09
Species
Human
AA Sequence
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