Recombinant human NCALD protein

Neurocalcin-delta, also known NCALD, is a member of the neuronal calcium sensor (NCS) family. NCALD is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. This protein is thought to be a regulator of signal transduction by G protein-coupled receptor. Recombinant human NCALD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Neurocalcin-delta, MGC33870, MGC74858, Neurocalcin delta

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

24.4 kDa (213aa) confirmed by MALDI-TOF

Additionnal Information

NCALD, Neurocalcin-delta, MGC33870, MGC74858, Neurocalcin delta, ATGP0895-10 µg, ATGP0895-20 µg, ATGP0895-50 µg, ATGP0895-100 µg, ATGP0895-250 µg, ATGP0895-500 µg, ATGP0895-1 mg, ATGP0895-10, ATGP0895-20, ATGP0895-50, ATGP0895-100, ATGP0895-250, ATGP0895-500, ATGP0895-1

References & Citations

Burqoyne RD., et al. (2007) Nat Rev Neurosci. 8 (3) :182-93.; ; Burqoyne RD., et al. (2004) Trends Neurosci. 27 (4) : 203-9

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 100 mM NaCl

Scientific Category

Neuroscience

NCBI Accession Number

NP_001035720

Uniprot Accession Number

P61601

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK IYGNFFPYGD ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISKAE MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA GQF