Recombinant human E2-25K/UBE2K protein

Ubiquitin-conjugating enzyme E2 K isoform 1, also known as uBE2K, is a member of the ubiquitin-conjugating enzymes family. These enzymes participate in a variety of cellular processes, including selective protein degradation, DNA repair, cell cycle control, and sporulation. In this mechanism, the ATP-coupled activation and subsequent ligation of ubiquitin are catalyzed by separate enzymes functionally linked by ubiquitin carrier protein uBC1. Also, uBE2K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-beta, huntingtin, and MHC-heavy chain proteins. Recombinant human uBE2K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ubiquitin-conjugating enzyme E2 K isoform 1, E2-25K, HIP2, HYPG, LIG, UBC1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

26.5 kDa (236aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

Ubiquitin-conjugating enzyme E2 K isoform 1, Ubiquitin conjugating enzyme E2 K isoform 1, UBE2K, UBC1, UBC 1, LIG, HYPG, HIP2, E2-25K, E225K, E2 25K, ATGP0894-10 µg, ATGP0894-20 µg, ATGP0894-50 µg, ATGP0894-100 µg, ATGP0894-250 µg, ATGP0894-500 µg, ATGP0894-1 mg, ATGP0894-010, ATGP0894-020, ATGP0894-050, ATGP0894-100, ATGP0894-250, ATGP0894-500, ATGP0894-01M

References & Citations

Chen Z., et al. (1990) J Biol Chem. 264:21835-21742.; ; Pichler A., et al. (2005) Nat Struct Mol. 12:264-269.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol, 50 mM NaCl

Scientific Category

Ubiquitination

NCBI Accession Number

NP_005330

Uniprot Accession Number

P61086

Species

Human

AA Sequence

MANI AVQRIKREFK EVLKSEETSK NQIKVDLVDE NFTELRGEIA GPPDTPYEGG RYQLEIKIPE TYPFNPPKVR FITKIWHPNI SSVTGAICLD ILKDQWAAAM TLRTVLLSLQ ALLAAAEPDD PQDAVVANQY KQNPEMFKQT ARLWAHVYAG APVSSPEYTK KIENLCAMGF DRNAVIVALS SKSWDVETAT ELLLSN