Recombinant human E2-25K/UBE2K protein
Ubiquitin-conjugating enzyme E2 K isoform 1, also known as uBE2K, is a member of the ubiquitin-conjugating enzymes family. These enzymes participate in a variety of cellular processes, including selective protein degradation, DNA repair, cell cycle control, and sporulation. In this mechanism, the ATP-coupled activation and subsequent ligation of ubiquitin are catalyzed by separate enzymes functionally linked by ubiquitin carrier protein uBC1. Also, uBE2K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-beta, huntingtin, and MHC-heavy chain proteins. Recombinant human uBE2K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ubiquitin-conjugating enzyme E2 K isoform 1, E2-25K, HIP2, HYPG, LIG, UBC1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
26.5 kDa (236aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
Chen Z., et al. (1990) J Biol Chem. 264:21835-21742.; ; Pichler A., et al. (2005) Nat Struct Mol. 12:264-269.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol, 50 mM NaCl
Scientific Category
Ubiquitination
NCBI Accession Number
NP_005330
Uniprot Accession Number
P61086
Species
Human
AA Sequence
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