Recombinant human RAP1B protein
RAP1B belongs to a superfamily of RAS-like small GTP-binding proteins involved in cell signaling. It possesses intrinsic GTPase activity and is ubiquitously expressed in mammalian tissues. The putative effector domain of Ras proteins, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, is conserved in both Rap 1 proteins. Recombinant human RAP1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Ras-related protein Rap-1b, DKFZp586H0723, K-REV, RAL1B, RAP1B member of RAS oncogene family, GTP-binding protein smg p21B
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
22.6 kDa (201aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Awasthi A., et al. (2010) J Exp Med. 207 (9) :1923-38.; ; Schoenwaelder SM., et al. (2010) J Biol Chem. 285 (4) :2886-96.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM MES buffer (pH 5.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_056461
Uniprot Accession Number
P61224
Species
Human
AA Sequence
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