Recombinant human RAP1B protein

RAP1B belongs to a superfamily of RAS-like small GTP-binding proteins involved in cell signaling. It possesses intrinsic GTPase activity and is ubiquitously expressed in mammalian tissues. The putative effector domain of Ras proteins, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, is conserved in both Rap 1 proteins. Recombinant human RAP1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ras-related protein Rap-1b, DKFZp586H0723, K-REV, RAL1B, RAP1B member of RAS oncogene family, GTP-binding protein smg p21B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

22.6 kDa (201aa) confirmed by MALDI-TOF

Additionnal Information

RAP1B, ATGP0891-10 µg, ATGP0891-20 µg, ATGP0891-50 µg, ATGP0891-100 µg, ATGP0891-250 µg, ATGP0891-500 µg, ATGP0891-1 mg, ATGP0891-10, ATGP0891-20, ATGP0891-50, ATGP0891-100, ATGP0891-250, ATGP0891-500, ATGP0891-1

References & Citations

Awasthi A., et al. (2010) J Exp Med. 207 (9) :1923-38.; ; Schoenwaelder SM., et al. (2010) J Biol Chem. 285 (4) :2886-96.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM MES buffer (pH 5.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_056461

Uniprot Accession Number

P61224

Species

Human

AA Sequence

MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS KINVNEIFYD LVRQINRKTP VPGKARKKSS C