Recombinant human NUDT2/APAH1 protein

NuDT2 is a member of the MutT family of nucleotide pyrophosphatases, a subset of the larger NuDIX hydrolase family. This protein maintains homeostasis by using water to cleave the metabolite NuDT symmetrically back into its original ATP and AMP molecules. NuDT2 is also active towards other adenosine and diadenosine polyphosphates with four or more phosphate groups, but not towards diadenosine triphosphate. NuDT2 is involved in heat shock and metabolic stress by regulating intracellular dinucleoside polyphosphate concentrations. Recombinant NuDT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nudix hydrolase 2, Bis (5'-nucleosyl) -tetraphosphatase asymmetrical, nudix (nucleoside diphosphate linked moiety X) -type motif 2, Ap4A hydrolase 1, Ap4Aase, diadenosine tetraphosphatase, diadenosine 5',5''-P1, P4-tetraphosphate pyrophosphohydrolase, APAH1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

19 kDa (167aa) confirmed by MALDI-TOF

Additionnal Information

NUDT2, bis (5'-nucleosyl) -tetraphosphatase [asymmetrical], APAH1, MGC10404, ATGP0890-10 µg, ATGP0890-20 µg, ATGP0890-50 µg, ATGP0890-100 µg, ATGP0890-250 µg, ATGP0890-500 µg, ATGP0890-1 mg, ATGP0890-10, ATGP0890-20, ATGP0890-50, ATGP0890-100, ATGP0890-250, ATGP0890-500, ATGP0890-1

References & Citations

Swarbrick JD., et al. (2005) J Biol Chem. 280 (9) :8471-81.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_671702

Uniprot Accession Number

P50583

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MALRACGLII FRRCLIPKVD NNAIEFLLLQ ASDGIHHWTP PKGHVEPGED DLETALRETQ EEAGIEAGQL TIIEGFKREL NYVARNKPKT VIYWLAEVKD YDVEIRLSHE HQAYRWLGLE EACQLAQFKE MKAALQEGHQ FLCSIEA