Recombinant human GRAP2 protein

GRAP2, also known as GADS, is a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Recombinant human GRAP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

GRB2-related adapter protein 2, GADS, GRB2L, GRBLG, GrbX, Grf40, GRID, GRPL, Mona, P38

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

40 kDa (350aa) confirmed by MALDI-TOF

Additionnal Information

GRAP2, GRB2-related adapter protein 2, GADS, GRB2L, GRBLG, GrbX, Grf40, GRID, GRPL, Mona, P38, ATGP0888-10 µg, ATGP0888-20 µg, ATGP0888-50 µg, ATGP0888-100 µg, ATGP0888-250 µg, ATGP0888-500 µg, ATGP0888-1 mg, ATGP0888-10, ATGP0888-20, ATGP0888-50, ATGP0888-100, ATGP0888-250, ATGP0888-500, ATGP0888-1

References & Citations

Ludwig L., et al. (2009) Cancer Lett. 275 (2) :194-7.; ; Chang TW., et al. (2008) Oncogene. 27 (3) :332-8.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 30% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_004801

Uniprot Accession Number

O75791

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MEAVAKFDFT ASGEDELSFH TGDVLKILSN QEEWFKAELG SQEGYVPKNF IDIQFPKWFH EGLSRHQAEN LLMGKEVGFF IIRASQSSPG DFSISVRHED DVQHFKVMRD NKGNYFLWTE KFPSLNKLVD YYRTNSISRQ KQIFLRDRTR EDQGHRGNSL DRRSQGGPHL SGAVGEEIRP SMNRKLSDHP PTLPLQQHQH QPQPPQYAPA PQQLQQPPQQ RYLQHHHFHQ ERRGGSLDIN DGHCGTGLGS EMNAALMHRR HTDPVQLQAA GRVRWARALY DFEALEDDEL GFHSGEVVEV LDSSNPSWWT GRLHNKLGLF PANYVAPMTR