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Recombinant human GATE-16 protein

GABARAPL2, also known as ATG8, is involved in intra Golgi traffic and modulates intra Golgi transport through coupling between NSF activity and SNAREs activation. GABARAPL2 first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. Recombinant human GABARAPL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Gamma-aminobutyric acid receptor-associated protein-like 2, GABA (A) receptor-associated protein-like 2, ATG8, ATG8C, GATE-16, GATE16, GEF2, FLC3A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

15.8 kDa (137aa) confirmed by MALDI-TOF

Additionnal Information

GEF-2, GEF2, GEF 2, GATE-16, GATE16, GATE 16, Gamma-aminobutyric acid receptor-associated protein-like 2, Gamma aminobutyric acid receptor associated protein like 2, GABARAPL2, GABA (A) receptor-associated protein-like 2, GABA (A) receptor associated protein like 2, GABA type A receptor associated protein like 2, FLC3A, ATG8C, ATG8, ATGP0885-10 µg, ATGP0885-20 µg, ATGP0885-50 µg, ATGP0885-100 µg, ATGP0885-250 µg, ATGP0885-500 µg, ATGP0885-1 mg, ATGP0885-010, ATGP0885-020, ATGP0885-050, ATGP0885-100, ATGP0885-250, ATGP0885-500, ATGP0885-01M

References & Citations

Behrends C., et al. (2010) Nature. 466 (7302) :68-76.; ; Sengupta A., et al. (2009) J Biol Chem. 284 (41) :28319-31.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_009216

Uniprot Accession Number

P60520

Species

Human

AA Sequence

MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL VPSDITVAQF MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD EDGFLYVAYS GENTFGF

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