Recombinant human GATE-16 protein
GABARAPL2, also known as ATG8, is involved in intra Golgi traffic and modulates intra Golgi transport through coupling between NSF activity and SNAREs activation. GABARAPL2 first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1. Recombinant human GABARAPL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Gamma-aminobutyric acid receptor-associated protein-like 2, GABA (A) receptor-associated protein-like 2, ATG8, ATG8C, GATE-16, GATE16, GEF2, FLC3A
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
15.8 kDa (137aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Behrends C., et al. (2010) Nature. 466 (7302) :68-76.; ; Sengupta A., et al. (2009) J Biol Chem. 284 (41) :28319-31.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 20% glycerol
Scientific Category
Cancer
NCBI Accession Number
NP_009216
Uniprot Accession Number
P60520
Species
Human
AA Sequence
MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL VPSDITVAQF MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD EDGFLYVAYS GENTFGF
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