Recombinant human BPNT1 protein

BPNT1, also known as BPntase, PAP phosphatase or PIP (PAP-inositol-1, 4-phosphatase), is a member of the magnesium-dependent, lithium-sensitive phosphomono-esterase superfamily. using magnesium as a cofactor, BPNT1 catalyzes the conversion of PAPS (adenosine 3'-phosphate 5' phosphosulfate) to APS (adenosine 5'-phosphosulfate) and the conversion of PAP (3' (2') -phosphoadenosine 5' phosphate) to AMP (adenosine 5'-phosphate) . Expressed ubiquitously with highest levels in brain and kidney, BPNT1 is potently inhibited by lithium, a drug used for the treatment of manic depression and bipolar affective disorder, suggesting a possible role for BPNT1 in the etiology of mood disorders. Recombinant human BPNT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

3' (2') 5'-bisphosphate nucleotidase 1, 3' (2'), 5'-bisphosphate nucleotidase 1, PIP, PAP-inositol-1,4-phosphatase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

37.5 kDa (344aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

BPNT1, 3' (2') 5'-bisphosphate nucleotidase 1, 3' (2'), 5'-bisphosphate nucleotidase 1, PIP, PAP-inositol-1,4-phosphatase, ATGP0883-10 µg, ATGP0883-20 µg, ATGP0883-50 µg, ATGP0883-100 µg, ATGP0883-250 µg, ATGP0883-500 µg, ATGP0883-1 mg, ATGP0883-10, ATGP0883-20, ATGP0883-50, ATGP0883-100, ATGP0883-250, ATGP0883-500, ATGP0883-1

References & Citations

Shaltiel G., et al. (2002) Bipolar Disord. 4 (5) :302-6. ; ; Agam G., et al. (2003) J Psychiatr Res. 37 (5) :433-42.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 5mM DTT, 0.1M NaCl.

Scientific Category

Neuroscience

NCBI Accession Number

NP_006076

Uniprot Accession Number

O95861

Species

Human

AA Sequence

MASS NTVLMRLVAS AYSIAQKAGM IVRRVIAEGD LGIVEKTCAT DLQTKADRLA QMSICSSLAR KFPKLTIIGE EDLPSEEVDQ ELIEDSQWEE ILKQPCPSQY SAIKEEDLVV WVDPLDGTKE YTEGLLDNVT VLIGIAYEGK AIAGVINQPY YNYEAGPDAV LGRTIWGVLG LGAFGFQLKE VPAGKHIITT TRSHSNKLVT DCVAAMNPDA VLRVGGAGNK IIQLIEGKAS AYVFASPGCK KWDTCAPEVI LHAVGGKLTD IHGNVLQYHK DVKHMNSAGV LATLRNYDYY ASRVPESIKN ALVP