Recombinant human BDH2 protein

BDH2, also known as DHRS6, belongs to the short-chain dehydrogenases/reductases (SDR) family. This protein may play an important role in the peripheral utilization of 3-hydroxybutyrate. The cytoplasmic localization with its high ratio of oxidized NAD+, the NAD+ dependence and the kinetic parameters of BDH2 make it suitable to convert high levels of circulating 3-hydroxybutyrate into acetoacetate. Recombinant human BDH2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

3-hydroxybutyrate dehydrogenase type 2, DHRS6, EFA6R, FLJ13261, PRO20933, SDR15C1, uCPA-OR, uNQ6308

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

28.8 kDa (265aa) confirmed by MALDI-TOF

Additionnal Information

BDH2, 3-hydroxybutyrate dehydrogenase type 2, DHRS6, EFA6R, FLJ13261, PRO20933, SDR15C1, uCPA-OR, uNQ6308, ATGP0882-10 µg, ATGP0882-20 µg, ATGP0882-50 µg, ATGP0882-100 µg, ATGP0882-250 µg, ATGP0882-500 µg, ATGP0882-1 mg, ATGP0882-10, ATGP0882-20, ATGP0882-50, ATGP0882-100, ATGP0882-250, ATGP0882-500, ATGP0882-1

References & Citations

Guo K., et al. (2006) . J. Biol. Chem., 281: 10291-10297; ; Ito K., et al. (2006) . J. Mol. Biol., 355: 722-733.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_064524

Uniprot Accession Number

Q9BUT1

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGRLDGKVII LTAAAQGIGQ AAALAFAREG AKVIATDINE SKLQELEKYP GIQTRVLDVT KKKQIDQFAN EVERLDVLFN VAGFVHHGTV LDCEEKDWDF SMNLNVRSMY LMIKAFLPKM LAQKSGNIIN MSSVASSVKG VVNRCVYSTT KAAVIGLTKS VAADFIQQGI RCNCVCPGTV DTPSLQERIQ ARGNPEEARN DFLKRQKTGR FATAEEIAML CVYLASDESA YVTGNPVIID GGWSL