Recombinant human REF-1/APEX1 protein

APEX1, also known as REF1 or APE1 (Apurinic / apyrimidinic endonuclease), is responsible for the incision of DNA basic sites during base excision repair. It has also been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression. APEX1 is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents. Recombinant human APEX1 protein, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Apurinic/apyrimidinic endodeoxyribonuclease 1, APEX nuclease, APEN, multifunctional DNA repair enzyme 1, DNA- (apurinic or apyrimidinic site) endonuclease, AP endonuclease 1, APE-1, Redox factor-1, APE, APE1, APEX, APX, HAP1, REF1

Expression System

E.coli

Antigen Species

Human

Tag

T7-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.9 kDa (332aa) confirmed by MALDI-TOF

Additionnal Information

APE, APEX nuclease (multifunctional DNA repair enzyme) 1, APE, APE1, APEN, APX, HAP1, REF-1, REF1, ATGP0881-10 µg, ATGP0881-20 µg, ATGP0881-50 µg, ATGP0881-100 µg, ATGP0881-250 µg, ATGP0881-500 µg, ATGP0881-1 mg, ATGP0881-10, ATGP0881-20, ATGP0881-50, ATGP0881-100, ATGP0881-250, ATGP0881-500, ATGP0881-1

References & Citations

Hinz AK., et al. (2010) J Biol Chem. 285 (37) :28683-90.; ; Wu HH., et al. (2010) Oncogene. 29 (30) :4330-40.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

AAH19291.1

Uniprot Accession Number

P27695

Species

Human

AA Sequence

MPKRGK KGAVAEDGDE LRTEPEAKKS KTAAKKNDKE AAGEGPALYE DPPDQKTSPS GKPATLKICS WNVDGLRAWI KKKGLDWVKE EAPDILCLQE TKCSENKLPA ELQELPGLSH QYWSAPSDKE GYSGVGLLSR QCPLKVSYGI GEEEHDQEGR VIVAEFDSFV LVTAYVPNAG RGLVRLEYRQ RWDEAFRKFL KGLASRKPLV LCGDLNVAHE EIDLRNPKGN KKNAGFTPQE RQGFGELLQA VPLADSFRHL YPNTPYAYTF WTYMMNARSK NVGWRLDYFL LSHSLLPALC DSKIRSKALG SDHCPITLYL AL