Recombinant human REF-1/APEX1 protein
APEX1, also known as REF1 or APE1 (Apurinic / apyrimidinic endonuclease), is responsible for the incision of DNA basic sites during base excision repair. It has also been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression. APEX1 is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents. Recombinant human APEX1 protein, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
T7-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
36.9 kDa (332aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hinz AK., et al. (2010) J Biol Chem. 285 (37) :28683-90.; ; Wu HH., et al. (2010) Oncogene. 29 (30) :4330-40.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
AAH19291.1
Uniprot Accession Number
P27695
Species
Human
AA Sequence
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