Recombinant human AK4 protein

AK4 (adenylate kinase 4) is a member of the adenylate kinase family of enzymes. This protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Inherited mutations leading to AK4 deficiencies in erythrocytes have been implicated in hemolytic anemia. Recombinant human AK4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Adenylate kinase 4, AK3, AK3L1, AK3L2, MGC166959

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.4 kDa (243aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

AK4, Adenylate kinase 4, AK3, AK3L1, AK3L2, MGC166959, ATGP0880-10 µg, ATGP0880-20 µg, ATGP0880-50 µg, ATGP0880-100 µg, ATGP0880-250 µg, ATGP0880-500 µg, ATGP0880-1 mg, ATGP0880-10, ATGP0880-20, ATGP0880-50, ATGP0880-100, ATGP0880-250, ATGP0880-500, ATGP0880-1

References & Citations

Noma T., et al. (2001) Biochem J. 358 (1) :225-32. ; ; Dzeja PP., et al. (1998) Mol Cell Biochem. 184 (1-2) :169-82.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 2mM DTT, 0.1M NaCl.

Scientific Category

Metabolism

NCBI Accession Number

NP_982289

Uniprot Accession Number

P27144

Species

Human

AA Sequence

MASKLLRAVI LGPPGSGKGT VCQRIAQNFG LQHLSSGHFL RENIKASTEV GEMAKQYIEK SLLVPDHVIT RLMMSELENR RGQHWLLDGF PRTLGQAEAL DKICEVDLVI SLNIPFETLK DRLSRRWIHP PSGRVYNLDF NPPHVHGIDD VTGEPLVQQE DDKPEAVAAR LRQYKDVAKP VIELYKSRGV LHQFSGTETN KIWPYVYTLF SNKITPIQSK EAY