Recombinant human RAB7A protein

Ras-related protein Rab-7a, also known as RAB7A, is a member of the Ras superfamily of small Rab GTPases. RAB7A associates with the RIPL effector protein to control membrane trafficking from early to late endosome and to lysosomes. RAB7A has also been shown to regulate growth receptor endocytic trafficking, degradation, maturation of phagosome and autophagic vacuoles. Recombinant human RAB7A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ras-related protein Rab-7a, RAB7, RAB7A member RAS oncogene family, CMT2B, Charcot-Marie-Tooth neuropathy 2B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

25.6 kDa (227aa) confirmed by MALDI-TOF

Additionnal Information

Rab7a, Ras-related protein Rab-7a, RAB7, ATGP0877-10 µg, ATGP0877-20 µg, ATGP0877-50 µg, ATGP0877-100 µg, ATGP0877-250 µg, ATGP0877-500 µg, ATGP0877-1 mg, ATGP0877-10, ATGP0877-20, ATGP0877-50, ATGP0877-100, ATGP0877-250, ATGP0877-500, ATGP0877-1

References & Citations

Feng Y., et al. (1995) J Cell Biol. 131:1435-52.; ; Barbero P., et al. (2002) J Cell Biol. 156:511-8.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 100 mM NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_004628.4

Uniprot Accession Number

P51149

Species

Human

AA Sequence

MTSRKKVLLK VIILGDSGVG KTSLMNQYVN KKFSNQYKAT IGADFLTKEV MVDDRLVTMQ IWDTAGQERF QSLGVAFYRG ADCCVLVFDV TAPNTFKTLD SWRDEFLIQA SPRDPENFPF VVLGNKIDLE NRQVATKRAQ AWCYSKNNIP YFETSAKEAI NVEQAFQTIA RNALKQETEV ELYNEFPEPI KLDKNDRAKA SAESCSC