Recombinant human Myoglobin protein

Myoglobin, mitochondrial precursor, also known as MB, is a cytosolic oxygen-binding protein responsible for the storage and diffusion of oxygen within myocytes. Expression of myoglobin is highest in skeletal and cardiac muscle. MB may serve a variety of functions in muscular oxygen supply, such as oxygen storage, facilitated diffusion, and myoglobin-mediated oxidative phosphorylation. Recombinant human MB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Myoglobin, PVALB

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

19.3 kDa (174aa) confirmed by MALDI-TOF

Additionnal Information

Myoglobin, Myoglobin, PVALB, ATGP0875-10 µg, ATGP0875-20 µg, ATGP0875-50 µg, ATGP0875-100 µg, ATGP0875-250 µg, ATGP0875-500 µg, ATGP0875-1 mg, ATGP0875-10, ATGP0875-20, ATGP0875-50, ATGP0875-100, ATGP0875-250, ATGP0875-500, ATGP0875-1

References & Citations

Jeffrevs A J., et al. (1984) Nucleic Acids Res. 12:3235-3243.; ; Qiu Y., et al. (1998) J Biol Chem. 273:23426-23432.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Scientific Category

Cancer

NCBI Accession Number

NP_976312.1

Uniprot Accession Number

P02144

Species

Human

AA Sequence

MGLSDGEWQL VLNVWGKVEA DIPGHGQEVL IRLFKGHPET LEKFDKFKHL KSEDEMKASE DLKKHGATVL TALGGILKKK GHHEAEIKPL AQSHATKHKI PVKYLEFISE CIIQVLQSKH PGDFGADAQG AMNKALELFR KDMASNYKEL GFQG