Recombinant human Myoglobin protein
Myoglobin, mitochondrial precursor, also known as MB, is a cytosolic oxygen-binding protein responsible for the storage and diffusion of oxygen within myocytes. Expression of myoglobin is highest in skeletal and cardiac muscle. MB may serve a variety of functions in muscular oxygen supply, such as oxygen storage, facilitated diffusion, and myoglobin-mediated oxidative phosphorylation. Recombinant human MB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Myoglobin, PVALB
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
19.3 kDa (174aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Jeffrevs A J., et al. (1984) Nucleic Acids Res. 12:3235-3243.; ; Qiu Y., et al. (1998) J Biol Chem. 273:23426-23432.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl
Scientific Category
Cancer
NCBI Accession Number
NP_976312.1
Uniprot Accession Number
P02144
Species
Human
AA Sequence
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