Recombinant human UCHL3 protein

Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as uCHL3, is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. uCHL3 play a role in the regulation of neuronal development and spermatogenesis and have been implicated in neurodegenerative diseases. uCHL3 protein is 54% identical to that of uCHL1. Recombinant human uCHL3 protprot, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 1,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5 at 37C.

Product Name Alternative

UCH-L3, ubiquitin thioesterase L3, ubiquitin carboxyl-terminal hydrolase isozyme L3, ubiquitin carboxyl-terminal esterase L3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 1,500pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0pmole of ubiquitin-AMC per minute at pH 7.5 at 37C.

Molecular Weight

28.3 kDa (250aa) confirmed by MALDI-TOF

Additionnal Information

HUMAN UCHL3, Ubiquitin carboxyl terminal esterase L3, Ubiquitin carboxyl terminal hydrolase isozyme L3, Ubiquitin carboxyl-terminal esterase L3, Ubiquitin carboxyl-terminal hydrolase isozyme L3, Ubiquitin thioesterase L3, Ubiquitin thiolesterase, Ubiquitin thiolesterase L3, UCH L3, UCHL3, UCH-L3, UCHL3 HUMAN, UCHL3_HUMAN, UCHL3HUMAN, ATGP0872-10 µg, ATGP0872-20 µg, ATGP0872-50 µg, ATGP0872-100 µg, ATGP0872-250 µg, ATGP0872-500 µg, ATGP0872-1 mg, ATGP0872-010, ATGP0872-020, ATGP0872-050, ATGP0872-100, ATGP0872-250, ATGP0872-500, ATGP0872-01M

References & Citations

Kwon J., et al. (2007) Exp Anim. 56:71-7.; ; Leroy E., et al. (1998) Nature. 395:451-2.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Ubiquitination

NCBI Accession Number

NP_005993

Uniprot Accession Number

P15374

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA