Recombinant human ACO1 protein
ACO1 (aconitate hydratase) plays a role as an iron sensor. This enzyme catalyzes the stereo-specific isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid cycle, a non-redox-active process. Recombinant human ACO1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Cytoplasmic aconitate hydratase, ACONS, IREB1, IREBP, IREBP1, IRP1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
100.8 kDa (912aa)
Additionnal Information
References & Citations
Vashisht A.A., et al (2009) Science 326:718-721; ; Salahudeen A.A., et al (2009) Science 326:722-726;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 100 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_002188
Uniprot Accession Number
P21399
Species
Human
AA Sequence
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items